Comparative Evaluation of Antimicrobial
Efficacy of Chlorhexidine, MTAD and
Chitosan as Root Canal Irrigant
against Enterococcus faecalis
Published: December 1, 2018 | DOI: https://doi.org/10.7860/JCDR/2018/36191.12368
Bhavani Srikrishna Pillai, Manavalan Madhana Madhubala, Arumugam Velmurugan, Vijay L Amiritharaj, Gangadharan Rajaraman
1. Associate Professor, Department of Conservative Dentistry and Endodontics, Rajah Muthiah Dental College and Hospital, Chidambaram, Tamil Nadu, India.
2. Associate Professor, Department of Conservative Dentistry and Endodontics, Sri Ramaswamy Memorial Dental College and Hospital, Chennai, Tamil Nadu, India.
3. Associate Professor, Department of Conservative Dentistry and Endodontics, Rajah Muthiah Dental College and Hospital, Coimbatore, Tamil Nadu, India.
4. Associate Professor, Department of Conservative Dentistry and Endodontics, Sri Ramaswamy Memorial Dental College and Hospital, Chennai, Tamil Nadu, India.
5. Associate Professor, Department of Conservative Dentistry and Endodontics, Rajah Muthiah Dental College and Hospital, Chidambaram, Tamil Nadu, India.
Correspondence
Dr. Manavalan Madhana Madhubala,
Flat No. 1c, Subham Lara Apartments, Kumananchavady Junction, Poonamalle High Road, Chennai-56, Tamil Nadu, India.
E-mail: dentistmadhana@yahoo.com
Introduction: Endodontic irrigants play a role in disinfection of root canal. Inspite of wider options on selection of irrigant, there is always a search on ideal antibacterial irrigant against recurrent infections.
Aim: To comparatively evaluate the antibacterial efficacy of chlorhexidine, Mixture of Tetracycline, Acid and Detergent (MTAD) and Chitosan against Enterococcus faecalis when used as a root canal irrigant.
Materials and Methods: The bacterial E. faecalis culture was grown overnight in Brain Heart Infusion (BHI) broth and inoculated in Mueller-Hinton agar plates. The root canal irrigants were divided into four groups as follows. (Group I)-4% Sodium hypochlorite solutions; (Group II)-2% Chlorhexidine (CHX); (Group III)-MTAD; (Group IV)-2% Chitosan. Bacterial inhibition was assessed using agar well diffusion method. All four study irrigants were added to respective wells in agar plate (n=10) and incubated at 37°C for 24 hour. Diameter of bacterial inhibition zone around each well was recorded. The results obtained were statistically evaluated using one-way ANOVA test and the intergroup comparison was done using student’s t-test.
Results: All the materials had statistically significant difference in zone of bacterial inhibition when compared to other materials. Based on the mean diameters, 4% sodium hypochlorite had the least zone of inhibition and MTAD had the highest zone of inhibition. 2% Chitosan polymer had greater zone of inhibition than 4% sodium hypochlorite, but less than 2% CHX and MTAD.
Conclusion: MTAD showed the highest antibacterial efficacy against Enterococcus faecalis followed by CHX and Chitosan.
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